Cytology & Histology

Histological Diagnosis

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Histological diagnosis can be defined as the microscopic examination of a biopsy or surgical specimen to study the manifestations of disease. In histological diagnosis, the tissue is taken off from the body by surgery, autopsy or biopsy then it is fixed over the glass slide as a method of stabilization and stained with dyes followed by examination under the microscope. The fixation is done to prevent tissue decay. Trained physicians, frequently licensed pathologists, are the personnel who perform histological diagnosis and provide diagnostic information based on their observations. Fixation, processing, and sectioning are the first three steps towards getting a diagnosis.